Effect of Fluorraton Growth Regulator and Male cultivar on the Protein pattern of Offshoots Date Palm Phoenix dactylifera L. Barhi resulted from tissue culture
Abstract
This study was conducted in the private orchards in the north of Basra Governorate. 18 female trees of date palm Barhi cultivar were selected that resulting from tissue culture. It was pollenated with the two male cultivars, the Gahnami and the male resulting from seeds. The study also included concentrations of Fluorraton prepared from the aqueous solution of the suspension. After the fruits reached the Kimri stage, the protein pattern of the fruits was studied. The results of the study showed that there are differences between each parameter or conditions of the study, where the specifications of the protein bands for all parameters differed. Also, all the fruits of the date palm-Barhi cultivar without treatments have participated in the presence of five protein bands and have shared the same molecular weight of the first protein band, which amounted to between 243.103 - 279.310 kD. It also shared the same molecular weight of the second protein band, which recorded a molecular weight between 172.915-183.221 kD. There were also differences between the study parameters in the number, locations and specifications of protein bands on polyacryl amide gel, the number of protein packs ranged between (5-7) protein bands depending on the male cultivar and fluoraton concentration, Five protein bands were in each of the treatment of the Ghanami Akhder cultivar and the seed cultivar and only six protein packs for the treatment (Ghanami Akhder + fluoraton 0.3 g.l-1). There are some unique treatments recording seven protein packages, namely three treatments (Ghanami Akhder + fluoraton 0.6 g.l-1) and (seed culivar + fluoraton 0.3 g.l-1) and (seedcultivar + fluoraton 0.6 g.l-1) and it seems that these three coefficients have affected the process of gene expression of the fruits of the Barhi cultivar and caused an increase in the number of protein bands.
